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You Searched For: Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Product Image-786-225

Total protein extraction kit, TPE™

Catalog Number: (786-225)
Supplier: G-Biosciences
Description: Total Protein Extraction (TPE™) Kit is a universal lysis system for the solubilisation of total proteins from animal, plant, yeast, bacteria, and other biological samples. The kit contains ready to use buffers for extracting total protein from cells and tissues for SDS-PAGE analysis.
UOM: 1 * 1 KIT
Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Catalog Number: (PIER88216)
Supplier: Thermo Fisher Scientific
Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
UOM: 1 * 1 items

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Product Image-786-791

Immobilised Pepsin

Catalog Number: (786-791)
Supplier: G-Biosciences
Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
UOM: 1 * 1 items
Product Image-786-790

Immobilised Papain

Catalog Number: (786-790)
Supplier: G-Biosciences
Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
UOM: 1 * 1 items
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Catalog Number: (PIER20308)
Supplier: Thermo Fisher Scientific
Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
UOM: 1 * 1 KIT
Product Image-PIER85160

Albumin depletion kit, Pierce™

Catalog Number: (PIER85160)
Supplier: Thermo Fisher Scientific
Description: Pierce™ Albumin Depletion Kit improves serum component analysis by rapidly removing abundant albumin protein from serum samples. The albumin depletion resin provided in the kit is a high capacity, immobilised Cibacron Blue dye agarose resin, which binds a variety of species-specific albumins.
UOM: 1 * 1 KIT
Product Image-PIER89871

In-Gel tryptic digestion kit, Pierce™

Catalog Number: (PIER89871)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ In-Gel Tryptic Digestion Kit provides a complete set of reagents for digesting and recovering peptide fragments from approximately 150 stained protein bands excised from SDS-PAGE (polyacrylamide gel plugs).
UOM: 1 * 1 KIT
Product Image-PIER89875

Albumin/IgG removal kit, Pierce™

Catalog Number: (PIER89875)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.
UOM: 1 * 1 KIT
Product Image-PIER89895

Pierce™ In-solution Tryptic Digestion and Guanidination Kit

Catalog Number: (PIER89895)
Supplier: Thermo Fisher Scientific
Description: The In-Solution Tryptic Digestion and Guanidination Kit contains proteomics grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.
UOM: 1 * 1 KIT
Product Image-PIER89805

Glycoprotein Isolation Kit, Cona And WGA, Pierce™

Supplier: Thermo Fisher Scientific
Description: The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Product Image-PIER28321

Detergent solutions, Surfact-Amps™

Supplier: Thermo Fisher Scientific
Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.

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Product Image-786-248

Protein fractionation kit, FOCUS™ cytoplasmic and nuclear proteins

Catalog Number: (786-248)
Supplier: G-Biosciences
Description: FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit for the fractionation of nuclear and cytoplasmic proteins from cells and tissues.
UOM: 1 * 1 KIT
Product Image-786-792

Immobilised Trypsin

Catalog Number: (786-792)
Supplier: G-Biosciences
Description: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
UOM: 1 * 1 items
Product Image-PIER77610

Carrier proteins, maleimide-activated carrier proteins, Imject™ Pierce™

Catalog Number: (PIER77610)
Supplier: Thermo Fisher Scientific
Description: Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.
UOM: 1 * 1 SET
Product Image-PIER89801

Mitochondria Isolation Kit for Tissue

Catalog Number: (PIER89801)
Supplier: Thermo Fisher Scientific
Description: The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.
UOM: 1 * 1 KIT
Product Image-786-232

FOCUS™ Protein Alkylation Kit

Catalog Number: (786-232)
Supplier: G-Biosciences
Description: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
UOM: 1 * 1 KIT
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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