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You Searched For: Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Product Image-786-253

Protein fractionation kit, FOCUS™ Glycoprotein

Catalog Number: (786-253)
Supplier: G-Biosciences
Description: FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.
UOM: 1 * 1 KIT
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Detergent removal system, DetergentOUT™ Tween®

Supplier: G-Biosciences
Description: DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

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Product Image-786-246

Proteome kits, mammalian proteome, FOCUS™

Catalog Number: (786-246)
Supplier: G-Biosciences
Description: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
UOM: 1 * 1 KIT
Image Unavailable-ENZOBMLKI1050300

Fluor-de-Lys® Developer I concentrate

Catalog Number: (ENZOBMLKI1050300)
Supplier: ENZO LIFE SCIENCES
Description: FLUOR DE LYS® Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate. An appropriate FLUOR DE LYS® family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS® Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins).
UOM: 1 * 300 µl

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Product Image-PIER88279

Mouse embryonic fibroblast isolation kit, Pierce™

Catalog Number: (PIER88279)
Supplier: Thermo Fisher Scientific
Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
UOM: 1 * 1 KIT
Product Image-PIER90067

Protein quantitation reagent, TMTzero™ label reagent

Catalog Number: (PIER90067)
Supplier: Thermo Fisher Scientific
Description: Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.
UOM: 1 * 1 KIT
Product Image-786-154

Detergent removal system, DetergentOUT™ GBS10

Supplier: G-Biosciences
Description: Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Product Image-786-018

Protein fractionation kit, FOCUS™ Global Fractionation

Catalog Number: (786-018)
Supplier: G-Biosciences
Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
UOM: 1 * 1 KIT
Product Image-786-255

Protein enrichment kit, FOCUS™ PhosphoRich™

Supplier: G-Biosciences
Description: FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotein binding resin with a binding capacity of ~20 mg phosphorylated ovalbumin per column. The resin columns supplied with the kit can be reused, if regenerated and stored properly. FOCUS™ PhosphoRich™ is suitable for 2D gel electrophoresis and for proteomics and cell signalling studies.

Product Image-786-274

Antibody F(ab)₂ fragmentation kits

Supplier: G-Biosciences
Description: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.

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SDS detection and estimation reagent kit

Catalog Number: (786-129)
Supplier: G-Biosciences
Description: A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.
UOM: 1 * 1 KIT
Product Image-786-128

Detergent removal systems, OrgoSol DetergentOUT™

Supplier: G-Biosciences
Description: OrgoSol DetergentOUT™ is suitable for removal of detergents from protein solutions, including hydrophobic protein solutions. Compatible with all detergent types, its performance is not dependent on the concentration of detergents in the solution. It is highly flexible and can be used to process small and large sample volumes.

Product Image-PIER89821

Complete bacterial protein extraction reagent, B-PER™

Supplier: Thermo Fisher Scientific
Description: B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble Protein Extraction and inclusion body purification from bacterial cell lysates.

Product Image-786-273

Antibody fab fragmentation kit

Supplier: G-Biosciences
Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.

Product Image-PIER90051

Pierce™ EndoProteinase Lys-C Protease, MS Grade

Catalog Number: (PIER90051)
Supplier: Thermo Fisher Scientific
Description: Endoproteinase Lys-C protease is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.
UOM: 1 * 20 µG
Product Image-ENZOENZ450160100

EpiXtract® Nuclear protein isolation kit

Catalog Number: (ENZOENZ450160100)
Supplier: ENZO LIFE SCIENCES
Description: Rapid, reliable method to isolate nuclear proteins in mammalian cells and tissues.
UOM: 1 * 1 KIT

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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