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You Searched For: Protein Expression Reagents

Since a successful protein expression is dependent on an efficient proteomic workflow, the reagents are designed to address all needs including induction of protein expression, cell lysis/extraction, capture, and purification. These substances are suitable for both small-scale and large-scale studies. Compatible with automated systems, the protein expression reagents enable regulated processing without monitoring the culture or adding any inducers during cell growth. A range of more specific reagents are available to fit any procedure.

Since a successful protein expression is dependent on an efficient proteomic workflow, the reagents are designed to address all needs including induction of protein expression, cell lysis/extraction, capture, and purification. These substances are suitable for both small-scale and large-scale studies. Compatible with automated systems, the protein expression reagents enable regulated processing without monitoring the culture or adding any inducers during cell growth. A range of more specific reagents are available to fit any procedure.


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TriLink® CleanCap® FLuc mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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TriLink® CleanCap® EPO mRNA (5moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko <em>et al.</em> showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.

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ALiCE for Research

Supplier: LENIOBIO
Description: From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free protein expression products with radical results for every production setup.

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TriLink® CleanCap® FLuc mRNA

Supplier: TRILINK BIOTECHNOLOGIES
Description: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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TriLink® CleanCap® M6 EPO mRNA (N1MePsU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: <p>EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.</p>

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TriLink® CleanCap® Cre mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites.

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TriLink® CleanCap® EGFP mRNA

Supplier: TRILINK BIOTECHNOLOGIES
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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TriLink® CleanCap® Beta gal mRNA (5moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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TriLink® CleanCap® Cas9 Nickase mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: <p>Cas9 Nickase mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.</p>

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TriLink® CleanCap® Cas9 mRNA

Supplier: TRILINK BIOTECHNOLOGIES
Description: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double stranded DNA cleavage.

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TriLink® CleanCap® OVA mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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TriLink® CleanCap® EGFP mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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TriLink® CleanCap® M6 EGFP mRNA (N1MePsU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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TriLink® CleanCap® Renilla Luc mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

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ALiCE for Scale-Up

Supplier: LENIOBIO
Description: ALiCE® - Linearly scalable eukaryotic cell-free protein expression. Scalable protein expression with ALiCE® works as effectively for assessment of future development as it does in the exploration phase. The same simple protocol applies across all scales. Add the DNA sequence and let ALiCE® make your protein.

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TriLink® CleanCap® mCherry mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma sp.</em> mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
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The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
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