You Searched For: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)

Description: BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of <i>E. coli</i>, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

RBC lysis buffer for human

Supplier: Thermo Fisher Scientific

Description: RBC lysis buffer for human, Thermo Fisher Scientific

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E. coli lysis buffer-II

Catalog Number: (J62613.AP)

Supplier: Thermo Fisher Scientific

Description: E. coli lysis buffer-II, Thermo Fisher Scientific

UOM: 1 * 500 mL

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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins

Catalog Number: (J61792.AP)

Supplier: Thermo Fisher Scientific

Description: Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific

UOM: 1 * 500 mL

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NP-40 lysis buffer with glycerol (2X)

Catalog Number: (J60143.AK)

Supplier: Thermo Fisher Scientific

Description: NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

UOM: 1 * 250 mL

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Triton® X-100 lysis buffer, pH 7,4

Supplier: Thermo Fisher Scientific

Description: Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

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NP-40 lysis buffer

Supplier: Thermo Fisher Scientific

Description: NP-40 lysis buffer, Thermo Fisher Scientific

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Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

Description: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.