DSP (Dithiobis(succinimidyl propionate)), Premium Grade, Pierce™

Supplier: Thermo Fisher Scientific

Synonyms: DTSSP, 3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester), DSP Crosslinker, Lomant’s reagent, 3,3'-Dithiodipropionic acid bis(N-succinimidyl Ester), Di(N-succinimidyl)-3,3'-dithiodipropionate, Sulfo-DSP, DTSP, 3,3'-Dithiodipropionic acid di(N-succinimidyl Ester)

PG82081
PIERPG82081EA 381 GBP
PIERPG82081
DSP (Dithiobis(succinimidyl propionate)), Premium Grade, Pierce™
DSP (Dithiobis(succinimidyl propionate))

Thermo Scientific Pierce Premium Grade DSP is our highest quality formulation of this popular amine-reactive, cleavable crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.


  • DSP—a thiol-cleavable, homobifunctional and amine-reactive crosslinking agent
  • High quality—identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity—enhanced level of testing and characterization compared to standard grade
  • Lot retention—ample supply of past lots retained to ensure future process testing
  • Change management—Change Control Notification (CCN) service
  • Consistent manufacture—batch-specific manufacturing documentation review


Compared to the standard grade product, Premium Grade DSP provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention, and (d) change control notification. DSP is dithiobis(succinimidyl propionate), an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm. DSP contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₄H₁₆N₂O₈S₂
MW: 404.41 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00042045
CAS Number: 57757-57-0

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Specification Test Results

1 g Sufficient For 2.47L of typical 1X (1mM) reaction solution
Product Line Pierce™
Labeling Method Chemical Labeling
Crosslinker Type Homobifunctional
Reactive Moiety NHS Ester
Spacer Cleavable, Long
Form Solid
Quantity 1 g / 10 g
Type Crosslinker
Solubility DMF, DMSO


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