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VWR®, Taq DNA polymerase

Supplier: VWR Chemicals
VWR®, <i>Taq</i> DNA polymerase
VWR®, <i>Taq</i> DNA polymerase
VWR®, <i>Taq</i> DNA polymerase
VWR®, <i>Taq</i> DNA polymerase

733-1300EA 54.8 GBP
733-1300 733-1301 733-1302 733-1303 733-1305 733-1307 733-1312 733-1313 733-1819 733-1820 733-1823 733-2009
VWR®, Taq DNA polymerase
Nucleic Acid Reagents End-point PCR Enzymes and Kits

VWR® Taq DNA Polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimisation. The enzyme is isolated from Thermus aquaticus, and has a molecular weight of approximately 94 kDa. VWR® Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a double strand 5' to 3' exonuclease activity. It leaves an A overhang, which makes the enzyme ideal for TA cloning.


  • Ideal choice for routine applications
  • High performance, thermostable DNA polymerase
  • Optimal for TA cloning


Taq DNA polymerase concentration: 5 Units/μl


10X Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween® 20
10X Extra Buffer: Tris-HCl pH 8,3; KCl, 15 mM MgCl₂, 1% Triton™ X-100
10X Mg-Free Key Buffer: Tris-HCl pH 8,5; (NH₄)₂SO₂, 1% Tween® 20
10X Mg-Free Extra Buffer: Tris-HCl pH 8,3; KCl, 1% Triton™ X-100


EU = Units


Delivery information: VWR® Taq DNA Polymerase is usually supplied with either or both Key Buffer and Extra Buffer. Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) minimising the need for optimisation of the Mg²⁺ concentration, or the annealing temperature in most primer-template systems. Extra Buffer is a traditional potassium (K⁺) buffer. Extra Buffer promotes high specificity, but careful optimisation of primer annealing temperatures and Mg²⁺ concentrations may be required.

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SPECIFICATIONS

Description Taq DNA Polymerase (733-1300) Taq DNA Polymerase (733-1301) Taq DNA Polymerase (733-1302) Taq DNA Polymerase (733-1303) Taq DNA Polymerase (733-1305) Taq DNA Polymerase (733-1307) Taq DNA Polymerase (733-1312) Taq DNA Polymerase (733-1313) Taq DNA Polymerase (733-1819) Taq DNA Polymerase (733-1820) Taq DNA Polymerase (733-1823) Taq DNA Polymerase (733-2009)
Environmentally Preferable
Format Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer Enzyme with/without buffer
Features Detergent free Detergent free Mg-free Mg-free Detergent free Mg-free
Hot start No No No No No No No No No No No No
Concentration 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl 5 U/µl
Units 250 500 1000 10000 1000 1000 1000 2500 2500 5000 10000 10000
Size 1 x 0.05 ml + 3 x 1.5 ml 1 x 0.1 ml + 3 x 1.5 ml 2 x 0.1 ml + 6 x 1.5 ml 3 x 0.667 ml + 18 x 5 ml 2 x 0.1 ml + 4 x 1.5 ml 2 x 0.1 ml + 4 x 1.5 ml 2 x 0.1 ml + 4 x 1.5 ml 5 x 0.1 ml + 10 x 1.5 ml 5 x 0.1 ml + 15 x 1.5 ml 10 x 0.1 ml + 9 x 5 ml 3 x 0.667 ml + 18 x 5 ml 3 x 0.667 ml + 12 x 5 ml
Buffer composition 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 10x Extra Buffer and 25 mM MgCl2
Detergent free		 10x Key Buffer and 25 mM MgCl2
Detergent free		 10x Key Buffer Mg-free and 25 mM MgCl2 10x Key Buffer Mg-free and 25 mM MgCl2 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2 10x Key Buffer, 10x Extra Buffer and 25 mM MgCl2
Detergent free		 10x Key Buffer Mg-free and 25 mM MgCl2
High fidelity No No No No No No No No No No No No
Amplicon size ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb ≤ 5kb
Processivity Standard Standard Standard Standard Standard Standard Standard Standard Standard Standard Standard Standard
Exonuclease activity 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’ 5’→3’
Product overhang 3'A 3'A 3'A 3'A 3'A 3'A 3'A 3'A 3'A 3'A 3'A 3'A
Storage conditions – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months) – 20 °C (long term), + 4 °C (6 months)
Application Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification Standard amplification

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on +353 1 88 22222
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at eurega_services@eu.vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service on +353 1 8822222.
Description Pk VWR Catalog Number Price Quantity
Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 250 EU 733-1300
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Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 500 EU 733-1301
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Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 1.000 EU 733-1302
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Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 2.500 EU 733-1819
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Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 5.000 EU 733-1820
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Taq DNA Polymerase, 10X Key Buffer (15 mM MgCl₂), 10X Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 10.000 EU 733-1303
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Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 1.000 EU 733-1312
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Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 2.500 EU 733-1313
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Taq DNA Polymerase, 10X MgCl₂-free Key Buffer, 25 mM MgCl₂ 10.000 EU 733-2009
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Taq DNA Polymerase, 10X MgCl₂-free Extra Buffer, 25 mM MgCl₂ 1.000 EU 733-1305
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Taq DNA Polymerase, 10X Tween-free Key Buffer (15 mM MgCl₂), 10X Triton-free Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 1.000 EU 733-1307
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Taq DNA Polymerase, 10X Tween-free Key Buffer (15 mM MgCl₂), 10X Triton-free Extra Buffer (15 mM MgCl₂), 25 mM MgCl₂ 10.000 EU 733-1823
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