Auramine O 85% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: 4,4'-(Iminomethylene)bis(N,N-dimethylaniline) hydrochloridePyoctanunum aureum4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloridePyoktanin YellowBasic Yellow 2Canary Yellow

M-Clarity™ quality level = MQ200

861030-25GEA 58.3 GBP
861030-25G
Auramine O 85% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®
Auramine O

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall. Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Formula: C₁₇H₂₂ClN₃
MW: 303,84 g/mol
Melting Pt: >250 °C (dec., lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00012484
CAS Number: 2465-27-2
EINECS: 219-567-2
UN: 3077
ADR: 6.1,III

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Specification Test Results

Appearance (Form); Powder and/or Chunks Conforms to Requirements
Infrared spectrum Conforms to Structure
Carbon 52.1 - 87.4 %
Nitrogen 10.7 - 18.0 %
Wavelength, c = 0.005 - 0.006 g/L; Water 429 - 435 nm
Extinction Coefficient ≥36000
Wavelength 367 - 373 nm
Extinction Coefficient ≥15000
Wavelength 249 - 255 nm
Extinction Coefficient ≥12000
Solubility (Turbidity), c = 1 mg/ml; 95% Ethanol Clear to Very Hazy
Biological Stain Commission Conforms
Dye Content ≥80 %

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