FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥90% (by HPLC), powder suitable for protein labeling, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: 5-isothiocyanatofluorescein5-FITCFluorescein Isothiocyanate, Isomer IFluorescein Isothiocyanate3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one

M-Clarity™ quality level = MQ200

F7250-100MGEA 36.4 GBP
F7250-100MG F7250-1G F7250-250MG F7250-500MG F7250-5G F7250-50MG
FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥90% (by HPLC), powder suitable for protein labeling, Sigma-Aldrich®
FITC (Fluorescein 5-isothiocyanate, Fluorescein isothiocyanate isomer I)

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525 nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitiser.


Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC). Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatisation reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitisation studies. Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC).

Formula: C₂₁H₁₁NO₅S
MW: 389,381 g/mol
Melting Pt: >360 °C (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00005063
CAS Number: 3326-32-7
EINECS: 222-042-0

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Specification Test Results

Appearance (Colour) Orange to Dark Orange
Appearance (Form) Powder
Solubility (Colour) Light Green-Yellow to Very Dark Green-Yellow and Faint Yellow to Dark Yellow
Solubility (Turbidity), 1 mg/ml, Acetone Clear
Proton NMR Spectrum Conforms to Structure
Degree of Coupling ≥85.0%
Purity (HPLC) ≥90%
Recommended Retest Period 2 years

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