Tween® 80 (Polysorbate), liquid, Cell Culture Grade

Supplier: MP Biomedicals
Warning

ICNA02194725.1EA 38.8 GBP
ICNA02194725.1
Tween® 80 (Polysorbate), liquid, Cell Culture Grade
Tween® 80 (Polysorbate)

Tweens® are a series of nonionic surfactants derived from sorbitan esters. They are soluble or dispersible in water but differ widely in organic and oil solubilities. Used as oil-in-water emulsifiers in pharmaceuticals, cosmetics, cleaning compounds, etc.
Tween® 80 has been widely used in biochemical applications including: solubilizing proteins, isolating nuclei from cells in culture selective protein extraction growing of tubercule bacilli, and emulsifying and dispersing substances in medicinal and food products. It has little or no activity as an anti-bacterial agent. It has been shown to have an adverse effect on the antibacterial effect of methyl paraben and related compounds. Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
Soluble/miscible in water to give a clear yellow solution; miscible with alcohol, dioxane, and ethyl acetate; practically insoluble in liquid paraffin and fixed oils (such as mineral oil).
Autoclaving of solutions is not recommended. Sterile filtering is suggested with a 0.22 micron filter. Tween® may need to be warmed to about 40 °C and alternated with portions of hot distilled water while being poured through the filter.
Store at Room Temperature (15-30 °C).


  • Cell Culture Reagent Grade
  • Concentration: ~75% oleic acid
  • Presentation: Liquid
  • Critical Micelle Concentration (CMC): 0.012 mM (13-15 mg/L)

Boiling Pt: 100 °C (lit.)
Melting Pt: –21 °C
Density: 1,08 g/mL
Flash Pt: >149 °C
Storage Temperature: Ambient
MDL Number: MFCD00082107
CAS Number: 9005-65-6
EINECS: 500-019-9
Merck Index: 13,07664

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Specification Test Results

Identity Test A Passes
Identity Test B Passes
Identity Test C Passes
Water Content ≤3.0%
Residue on Ignition ≤0.25%
Specific Gravity 1.06-1.09
Viscosity 300-500cps
Saponification Value 45-55

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